BSLT Toxicity Test Of Temulawak Ethanol Extract (Curcuma Xanthorrhiza) From Five Different Regions And Determination Of Curcumin And Xanthorhizol Compound Levels

Abstract

The BSLT toxicity test is a preliminary test to look for ingredients that have the potential to act as anticancer from natural ingredients. A natural ingredient that has the potential to be an anticancer candidate is ginger. The aim of this research is to determine the level of toxicity against Artemia salina and look for anticancer candidates from ginger originating from different regions based on their toxicity, establish quality standards and determine the levels of curcumin and xanthorrhizol from extracts that have the highest toxicity. This research made 50% ethanol extract of ginger from the Cirebon, Tembalang, Wonogiri, Jambi and Southwest Sumba areas using the kinetic maceration method. The fifth extract was subjected to a phytochemical screening test, toxicity test using the BSLT method. The ginger extract which had the highest toxicity was tested for non-specific parameters and determined the levels of curcumin and xanthorrhizol. The results of phytochemical screening showed positive flavonoids, saponins, quinones, triterpenoids. The LC50 results obtained from the five extract samples were 48.34 mg/L, 41.85 mg/L 64.58 mg/L, 105.32 mg/L, 82, 17mg/L. The results of determining non-specific parameters met the specified requirements and obtained curcumin and xanthorrhizol levels of 72.83 and 117.07 ppm. Based on these results, it can be concluded that the 50% ethanol extract of Javanese tumeric from Tembalang meets the extract quality requirements, contains curcumin and xanthorrhizol compounds and has cytotoxic activity against Artemia salina.